منابع مشابه
Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing.
In the human genome, the majority of protein-encoding genes are interrupted by introns, which are removed from primary transcripts by a macromolecular enzyme known as the spliceosome. Spliceosomes can constitutively remove all the introns in a primary transcript to yield a fully spliced mRNA or alternatively splice primary transcripts leading to the production of many different mRNAs from one g...
متن کاملRNA nanotechnology breakthrough for targeted release of RNA-based drugs using cell-based aptamers
Nucleic acids play different roles besides storing information and proteins coding. For example, single-stranded nucleic acids can fold into complicated structures with capability of molecular detection, catalyzing bioreactions and therapy. The development of RNA-based therapies has been rapidly progressed in the recent years. RNA aptamers are biomolecules with a size of 10 to 50 nm that can be...
متن کاملRNA-Based Tools for Nuclear Reprogramming and Lineage-Conversion: Towards Clinical Applications
The therapeutic potential of induced pluripotent stem cells (iPSCs) is well established. Safety concerns remain, however, and these have driven considerable efforts aimed at avoiding host genome alteration during the reprogramming process. At present, the tools used to generate human iPSCs include (1) DNA-based integrative and non-integrative methods and (2) DNA-free reprogramming technologies,...
متن کاملReprogramming human somatic cells to pluripotency using RNA
Somatic cells can be reprogrammed to a pluripotent stem-cell state by ectopic expression of defined proteins. However, existing reprogramming methods take several weeks, suffer from low efficiencies, and most use DNA-based vectors, which carry mutagenesis risks. Here, we describe efficient and rapid reprogramming of human cells using RNA. Within two weeks, fibroblasts from 7 adult patients, inc...
متن کاملReprogramming cellular behavior with RNA controllers responsive to endogenous proteins.
Synthetic genetic devices that interface with native cellular pathways can be used to change natural networks to implement new forms of control and behavior. The engineering of gene networks has been limited by an inability to interface with native components. We describe a class of RNA control devices that overcome these limitations by coupling increased abundance of particular proteins to tar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2010
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0111-18